- Viola – a shipwrecked young woman who disguises herself as a page named Cesario
- Sebastian – Viola's twin brother
- Duke Orsino – Duke of Illyria
- Olivia – a wealthy countess
- Malvolio – steward in Olivia's household
- Maria – Olivia's gentlewoman
- Sir Toby Belch – Olivia's uncle
- Sir Andrew Aguecheek – a friend of Sir Toby
- Feste – Olivia's servant, a jester
- Fabian – a servant in Olivia's household
- Antonio – a sea captain and friend to Sebastian
- Valentine and Curio – gentlemen attending on the Duke
- A Sea Captain – a friend to Viola
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